European Journal of Plant Pathology (2018) 150, 785-789

Duska Delic, Bachir Balech, Mariana Radulovic, Zorica Duric, Biljana Lolic, Monica Santamaria and Gordana Duric (2018)
Molecular identification of Tomato spotted wilt virus on pepper and tobacco in Republic of Srpska (Bosnia and Herzegovina)
European Journal of Plant Pathology 150 (3), 785-789
Abstract: Surveys for TSWV presence and distribution on vegetable, ornamental and tobacco plants were conducted during 2016 in open fields and greenhouses in different locations in Republic of Srpska, district of Bosnia and Herzegovina. One hundred and twelve samples were taken and preliminarily tested for TSWV presence with DAS-ELISA. Positive samples were double-checked in RT-PCR assays using specific primer sets and subsequently partial sequences of RdRp and N region genes were characterized. Serological and molecular results revealed twenty-seven TSWV positive samples (two peppers and twenty-five tobacco plants). Moreover, phylogenetic analyses showed that these isolates share the same gene pool and a similar evolutionary pattern mainly with other isolates present in Europe and Russia.
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Link to article at publishers website
Database assignments for author(s): Duska Delic, Zorica Duric

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orthotospovirus tomatomaculae	Green pepper/chilli (Capsicum)	Bosnia and Herzegovina
	Orthotospovirus tomatomaculae	Tobacco (Nicotiana tabacum)	Bosnia and Herzegovina